FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22345573
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG73650.021.586561120
CGTCTAG28050.018.6139951
TCGCCGG95150.018.285192120
TAGGACG81400.018.2841054
CGGTGGG95700.017.678585120
CGCCGTT37000.017.511864120
CGTATCC174100.016.816338120
GACGAGG111850.016.57421120
AGGACGT90600.016.3611985
TAGATCC222000.015.485027120
CCGACGG55550.015.228087120
CGTCAGA45350.015.2185662
GCGTCAG48650.015.1731411
GTCCTAC134150.014.5841171
GGACGTG101500.014.18915756
GTAGGAC138900.013.6101063
GGTACGA24250.013.60815318
CGACTTG53650.012.97311464
CGTACGT21200.012.73476310
TTGACGA54500.012.66068960
Produced by FastQC (version 0.11.2)