FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22345573
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1258720.5632972580295883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27850.023.0499921
CGTCAGA44850.022.7404291
TCTGACG40700.019.30831311
TAGGACG84650.017.7181914
AGGACGT91700.015.8326055
CGTACGT19250.015.581408510
CCGTACG21300.015.2083349
CTGACGC55350.015.06494512
CGTATCG17700.014.91237710
GGACGTG99000.014.7256836
CTAGACG28950.014.5064673
GGTACGA21950.014.48516418
GTCCTAC135700.014.32442951
TTGACGA53350.014.2794560
CGGCTAT42350.014.02257835
TGACGCC40250.014.00893613
TACGCGT30100.013.752735
GTAGGAC137000.013.7505873
GACGCCT41700.013.66688814
CTACGGT24400.013.27538413
Produced by FastQC (version 0.11.2)