FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22357683
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG77200.019.583704120
CGTCTAG29450.019.5644021
TAGGACG82450.017.9064124
TCGCCGG97950.017.885044120
CCGACGG53350.017.43046120
CGTCAGA44250.016.9542032
CGGTGGG95350.016.862644120
CGTATCC186650.016.714252120
CGCCGTT39550.016.686213120
GTCCTAC129950.016.026321
AGGACGT91900.015.4118845
GCGTCAG51250.015.2240471
GGACGTG98950.014.4332476
GACGAGG107600.013.71622120
GTAGGAC141050.013.6588013
TAATCGA21700.013.54875721
TTAGTCG23750.013.38888139
TAGATCC235750.013.258598120
CTAGACT126200.013.1254394
CTACGGT24400.013.03209313
Produced by FastQC (version 0.11.2)