FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22357683
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1268600.567411211617948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACG42600.021.40365211
CGTCAGA47700.020.6251351
CGTCTAG28000.020.5676441
TAGGACG79450.018.3477084
TACGCGT34850.016.8691315
CTGACGC57050.016.50813112
AGGACGT89600.016.001455
ACGCGTA36200.015.5754686
GTCCTAC134800.014.8192331
GTAGGAC136400.014.7332623
CTGGTCG41150.014.7226829
GGACGTG97050.014.7096816
CGACTTG53800.014.63801564
CTAGACG29000.014.2731413
TTGACGA53200.014.20638860
GTACTAG76600.014.0183091
TATGGCG97000.013.97646616
TAGTCGA23550.013.755375
CGTATCG17900.013.73993710
GACGACT57900.013.67532362
Produced by FastQC (version 0.11.2)