FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22398893
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG28950.019.0705381
TCTCGGG75700.018.624937120
CGTATCC188600.017.65526120
CGGTGGG97050.016.567694120
CCGACGG52250.016.534803120
TCGCCGG97400.016.384964120
TAGGACG79550.016.218924
CGCCGTT37500.015.8389635120
CGTCAGA44750.015.8238662
GCGTCAG50350.014.7790291
GACGAGG104900.014.469977120
AGGACGT88300.014.2719145
ATACCGT30350.014.2347856
GTCCTAC133950.014.1120941
GGTACGA24050.013.97094618
TGACGCC89050.013.878934120
TACGCGT33650.013.1968515
ACGCGTA34200.012.9832296
GTAGGAC138850.012.7929133
CGGCTAT42950.012.71369635
Produced by FastQC (version 0.11.2)