FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22398893
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1263330.5640144805370516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACG41450.023.01092711
CGTCTAG26350.022.7681851
CGTCAGA44450.019.975561
TAGGACG78300.018.4644014
CTGACGC55850.017.6149812
TACGCGT31050.016.8087965
ACGCGTA32500.016.2420376
AGGACGT87350.015.7272055
GTAGGAC136400.015.3933673
GTCCTAC130000.015.32158851
CTAGACG29950.015.0225743
CTACGGT25700.014.70526413
ATGCGAA29500.014.23488617
TTGACGA52350.014.20849660
CCGTAAC27950.014.1648229
GGACGTG95350.014.0289556
TACGGTG27400.014.01182914
TGACGCC41600.013.9876213
GATGCGA29500.013.82823816
CCGTACG21700.013.8217519
Produced by FastQC (version 0.11.2)