FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22418529
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGT42550.045.7185791
CGACGTA42000.045.6694892
TGTCGGT31550.040.96193393
ACGTATC46300.040.39967794
GGTCGAC48300.039.99505289
ACCGTAT42450.037.81468694
CACCGTA42950.037.3744793
GTCGACG51750.037.19661390
TGGTCGA52850.036.42436688
ATGTCGG41200.033.34881292
GTGATCG29750.032.92317691
GACGTAT59250.031.22531193
ACGCCGT37050.030.84233794
TAGATCG38750.030.71969688
GATGTCG47400.030.56521491
GTCGATC33950.029.8519592
ATCGCCG31750.028.92119694
TGCCGAT33450.028.87638388
TCACCGT60500.028.78132692
GATCGCG42600.028.42089390
Produced by FastQC (version 0.11.2)