FastQCFastQC Report
Mon 11 Dec 2017
Cast-M-ATAC-2-6_TAGGCATG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-M-ATAC-2-6_TAGGCATG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22418529
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1236960.5517578784941688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACG44550.023.8339211
CGTCAGA47150.021.6312521
CTGACGC58500.018.97093212
CGTCTAG26650.018.0097311
TGACGCC43600.016.7859613
CGACGAA20450.016.7210723
GGTACGA23050.016.65698418
GTACGAG24900.015.90120419
TAGGACG82150.015.847754
CTAGACG26250.015.0843023
TACGGTG26450.014.9684414
CGTATCG16950.014.86457210
GTCCTAC132950.014.7561781
GACGCCT45500.014.63422914
CTACGGT26400.014.08836413
TACGAGG28550.014.07842620
AACTGCG39850.014.0000437
AGGACGT91350.013.7262915
TATGGCG100400.013.68330616
TAGACCG27800.013.5960015
Produced by FastQC (version 0.11.2)