FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17565814
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT25850.018.800747120
CCGACGG39750.018.716955120
TCGCCGG78700.018.526022120
CGTATCC122750.018.281033120
CGGTGGG78150.018.272528120
TAGGACG80150.017.5953964
TCTCGGG60900.017.53692120
CGTCTAG22150.017.068741
GTCCTAC116000.015.8306281
AGGACGT87350.015.4563645
GACGAGG78500.015.439469120
GGACGTG93150.014.491996
TACGCGT24600.014.3914535
ATACCGT20500.014.3407096
CGTAATC19100.014.13578422
TAATCGA15000.013.99950221
TACACGG27800.013.5982635
TATGGCG90250.013.49513416
ACGCGTA24950.013.4662266
TAGATCC147000.013.428553120
Produced by FastQC (version 0.11.2)