FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17565814
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT370060.21067056727345512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG85300.018.5679284
ACGGACC40700.017.83522120
CGTATCG11550.017.65773610
CGTCTAG20350.016.5076941
AGGACGT92750.016.4942875
GTCCTAC111550.016.1329771
GTAGGAC122750.015.444513
GGACGTG98950.015.3998536
CGACTTG51150.015.38968164
GACGACT54150.015.28665862
TTGACGA54250.015.2584860
TACCGTA16800.014.6405857
ACGCGTA23100.014.5436946
TACACCG22150.014.35520655
TGACGAC58450.014.26468661
GTACCGT9700.014.22508056
GGTACGA16100.014.1590118
GTACGAG17950.014.03653819
CTACGGT18050.013.62514113
TACGCGT23700.013.4163645
Produced by FastQC (version 0.11.2)