FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17575503
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG78450.018.662003120
CCGACGG42100.018.242704120
CGCCGTT29950.018.030472120
TCTCGGG60050.017.985432120
CGTATCC119950.017.757814120
TAGGACG81100.016.7224864
CGGTGGG76150.015.837534120
CGTCTAG21600.015.8355951
GTCCTAC116500.015.0922831
AGGACGT88900.014.5786015
GACGAGG78100.014.5201845120
TACGCGT23200.014.483185
ACGCGTA23500.013.7865756
GGACGTG96250.013.33962256
TAGATCC153650.013.316289120
CCGTACG16900.012.7803469
CGTACGT16000.012.74928210
GGTACGA15600.012.69162818
TACAGTG166250.012.6319465
GGTCGGT35650.012.28542211
Produced by FastQC (version 0.11.2)