FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17575503
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT367710.20921734074979248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20300.020.9814821
ACGGACC41300.016.704235120
TAGGACG79550.016.516854
GTCCTAC115100.016.0006241
CGACTTG52100.015.68313564
TTGACGA52900.015.53474560
GGTACGA15100.015.49410418
GACGACT54350.015.45139662
CTAGACG23400.015.1272313
GTAGGAC121600.015.0977123
AGGACGT87200.014.9989495
CCGTACG16000.014.9977129
TGACGAC57450.014.93087661
CTACGGT20300.014.77451413
TTAGTCG15300.014.50775439
CGTACGT15300.014.50606310
TAATCGA16700.014.01023621
ACGACTT60400.013.70507563
CGACGAA14450.013.69990323
GTACGAG17250.013.56345619
Produced by FastQC (version 0.11.2)