FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17530912
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC120650.019.942049120
TCGCCGG74150.019.74383120
TCTCGGG58650.019.335106120
CCGACGG40900.018.337471120
TAGGACG80850.017.2934114
CGGTGGG72100.016.893261120
CGTCTAG21150.016.739651
ATACCGT22050.016.5986486
CGCCGTT25400.016.53549120
AGGACGT89550.015.20990855
GACGAGG82300.014.507947120
GTCCTAC117900.014.5056041
GTAGGAC117200.014.48984053
CTACGGT16150.014.48834613
TACGGTG17600.013.97648314
TTGACGA50650.013.85903960
GGACGTG94300.013.6797546
TAGATCC155500.013.58204120
TACCGTA20450.013.4961867
CGACTTG47750.013.44430564
Produced by FastQC (version 0.11.2)