FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17530912
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT362490.20677190097126721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG17500.021.9388031
TAGGACG82100.018.7068214
ACGGACC39600.018.329264120
AGGACGT88050.017.3745175
GTCCTAC112100.016.0541251
GGACGTG94750.015.8913866
TTGACGA50900.015.55575860
GTAGGAC125300.015.5130473
CGACTTG50450.014.29807864
TACACCG21450.014.2641145
GACGACT51500.014.09331662
TAGTCGA15900.013.960685
GTACTAG55550.013.9308181
TGACGAC56350.013.83835661
ACGTACG14050.013.66252537
GGTACGA17200.013.60221518
GACGTGG63750.013.5503597
GTACGAG19100.013.50651219
CGTATCG12950.013.43273710
TACACTG142650.013.0374265
Produced by FastQC (version 0.11.2)