FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17567474
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG73250.021.213583120
CGCCGTT25800.020.928816120
CGTATCC119400.020.249886120
TAGGACG82950.017.795134
AGGACGT88850.016.6809965
CCGACGG38750.016.256966120
CGTCTAG21050.016.2490771
GTCCTAC115650.015.1510641
CGGTGGG74200.015.120271120
TCTCGGG58200.015.050529120
CGACTTG49800.014.81852764
GGACGTG98000.014.755916
GACGAGG77800.014.574849120
TAGATCC160500.014.354169120
TTGACGA52600.014.25783460
ATACCGT19400.014.2275846
GTAGGAC119750.014.0309943
CTACGGT18850.014.00491613
CTAGACG22750.013.7160133
CCGTACG17800.013.1451799
Produced by FastQC (version 0.11.2)