FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17567474
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT367420.20914788318457167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20350.020.636111
TAGGACG81300.019.1125354
ACGGACC41200.018.490927120
AGGACGT90750.016.8588735
GTCCTAC110950.016.491751
TACGCGT20100.016.4172955
GGACGTG96800.015.7415986
GTAGGAC125300.015.6090183
ACGCGTA21350.014.89252956
ATTCGAA18400.014.6699432
CGTATCG11750.014.29493710
CGACTAA17500.014.05425610
CGACGAA14600.013.96961423
TCGTACG4751.0010353E-613.89307197
CGAATTG19550.013.50094822
TTCGTCG13900.013.37763328
CGGCTAT31450.013.35091535
TTGACGA50850.013.32968560
TCTAGAC94750.013.2335433
TATGGCG94700.013.17605316
Produced by FastQC (version 0.11.2)