FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17457923
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG76050.019.406038120
TAGGACG77350.018.0003284
CGTCTAG21050.017.3917371
CGTATCC128650.017.347391120
CGGTGGG76950.017.15201120
AGGACGT81900.016.4873245
TCTCGGG59700.016.480505120
TACGCGT21700.016.040575
CCGACGG42400.015.988715120
ATACCGT21050.015.964636
GACGAGG81100.015.682526120
TACCGTA18500.015.2439677
CGCCGTT28750.015.23302120
ACGCGTA23000.015.1329316
GGACGTG89100.014.95196156
TAGATCC166600.014.692178120
GTCCTAC111450.014.64718151
TTGACGA48600.014.567842560
CGTACGT14450.014.53152310
CCGTACG15700.014.1384519
Produced by FastQC (version 0.11.2)