FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17457923
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT364300.2086731623229178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG11350.021.66910210
ACGGACC40900.019.21595120
TAGGACG80150.017.815014
ACGCGTA22750.017.4047976
TACCGTA16750.017.1901387
CGTCTAG21650.017.1811181
ACCGTAT18800.016.9110648
TACGCGT23800.016.6371775
AGGACGT85250.015.412125
GTAGGAC116900.015.293943
TTGACGA50900.014.9663760
GTCCTAC110250.014.9648281
CTACGGT19250.014.95696613
ATTACGC15650.014.94894937
TATTACG15000.014.796267536
GGACGTG92500.014.3985146
CGTACGT12300.014.14273334
GACGACT51350.014.01792762
GGTCGGT34450.013.93008211
TACGGTG20700.013.9107714
Produced by FastQC (version 0.11.2)