FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17495012
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC135600.018.40481120
CCGACGG39950.016.668783120
TCGCCGG72850.016.552523120
CGCCGTT28050.016.25468120
ATACCGT19350.016.1264696
TACCGTA17550.016.0685087
CGTCTAG20400.015.8875241
CGGTGGG73800.015.851704120
TAGGACG76900.015.7652744
GTCCTAC117750.015.5974331
TCTCGGG59500.015.325841120
GACGAGG77600.015.07546120
AGGACGT85250.014.1505055
TAGATCC180050.013.794467120
TACGGTG16950.013.45155314
GGACGTG89950.013.00919156
GTAGGAC117650.012.9578933
CTACAGT156300.012.9019934
TACAGTG164000.012.7717925
CCGTACG17050.012.6670969
Produced by FastQC (version 0.11.2)