FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17495012
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT367860.21026564600241487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20800.023.650291
ACGGACC42700.021.920025120
CGTATCG12650.017.07116510
TAGGACG80900.017.0553384
GTAGGAC122450.016.020323
AGGACGT88250.015.8387975
GGTACGA15000.015.19567318
GTCCTAC113800.014.3915091
TAATGCG17550.014.3566514
ACGTACG15100.014.30080937
TATGGCG93400.014.25779316
CGGCTAT33750.014.21829535
CTAGACG22250.014.0202433
GGACGTG95200.013.8612436
TTGACGA53550.013.77731360
CGTACGG15500.013.54445338
TACACGG27050.013.528315
CCGTGAT57200.013.21271827
GTACGAG17800.013.14236319
GACGACT54450.013.10962762
Produced by FastQC (version 0.11.2)