FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17588476
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT28400.020.280222120
CGTATCC139050.018.725721120
TCTCGGG58250.018.436432120
TCGCCGG76450.017.657322120
TAGGACG82900.017.3735644
CCGACGG42450.017.242569120
AGGACGT89900.015.8205235
CGGTGGG74000.015.566442120
GACGAGG80650.015.547542120
CGTCTAG20500.015.5150851
GTCCTAC112950.014.9828711
GTATAGT97350.014.2399411
GTAGGAC121400.014.236593
GGACGTG95300.014.1667396
TTGACGA50950.013.89572160
GCTCGAA23800.013.86458211
CGACTTG50250.013.49228964
CGAATTG21050.013.39669522
TGCTCGA24050.013.22149610
TAGATCC187500.013.183045120
Produced by FastQC (version 0.11.2)