FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17588476
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT369140.20987605748218324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG19700.022.8404351
ACGGACC43350.019.79301120
TAGGACG81350.018.8043614
AGGACGT87950.017.1886085
CGTATCG12400.016.44787410
GGACGTG92500.015.9525876
GTCCTAC114650.015.8030971
GTAGGAC120800.015.6429853
TACGCGT22400.015.5330235
ACGCGTA22150.015.1653366
CGACTTG49550.014.91564
TTGACGA52350.014.8958260
TACGGTG17800.014.82846914
CTACGGT18400.014.67095413
CCGTACG14550.014.430202533
CGTACGT14100.014.03984234
TAGTCGA14400.013.7476185
CGTAATC18900.013.64784622
AACTGCG29600.013.5775337
GGTACGA15100.013.50686518
Produced by FastQC (version 0.11.2)