FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17534310
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGT31100.046.35605291
CGACGTA31350.046.20300392
ACGTATC34050.040.34246494
ACCGTAT32200.039.91481494
GGTCGAC36950.039.20193589
GTCGACG37200.039.12039690
CACCGTA32500.038.91864893
TGGTCGA40700.035.59169888
ACGCCGT29650.035.32028694
TGTCGGT26900.035.13910393
GTCGATC22950.033.18665792
GACGTAT42250.032.51268493
TCACCGT43100.031.39814292
TAGATCG29200.030.74359188
ATGTCGG31250.030.6829692
GTGATCG20350.029.7410791
GATGTCG34150.029.27235291
CCGTCGA19050.029.2718391
TGCCGAT25250.029.08880488
GTGGACG39200.028.97095390
Produced by FastQC (version 0.11.2)