FastQCFastQC Report
Mon 11 Dec 2017
Cast-F-ATAC-2-5_GGACTCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCast-F-ATAC-2-5_GGACTCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17534310
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT352310.20092607008773086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG5350.022.43050299
ACGGACC42800.020.329035120
CGTCTAG19400.018.865081
TAGGACG82500.016.6539424
TACACCG23750.014.9047235
AGGACGT90900.014.7849435
GTCCTAC115100.014.6474331
CGTATCG11900.014.61931810
GTACGAG17250.014.606519
GGTACGA16450.014.58734718
GTAGGAC124450.014.3664083
AACTGCG30850.014.1953547
TACGCGT20800.014.1341045
GGACGTG98000.013.8951976
ACGCGTA20950.013.7442856
CTACGGT17750.013.18055313
TATGGCG96050.013.05362716
TTTCGAC18950.012.98065446
TACGAGG20450.012.90759620
TGCACCG29500.012.8130955
Produced by FastQC (version 0.11.2)