FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20734238
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT483110.23300108738020658Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG54200.023.690351120
CGTATCC133800.022.511509120
CGTCTAG27700.022.31411
TACGGTG26400.020.90785614
CTACGGT27050.020.62724713
CGCCGTT26250.020.343128120
TCGCCGG75450.020.040026120
CGGTGGG66550.019.474339120
CTAGACG33050.018.8835583
TAGGACG64600.018.5788764
CCTACGG31150.017.91226412
TTGACGG32350.017.80600731
GCGAATT30000.017.79907619
TATTACG27750.017.51273236
TCCGCAA33000.017.27341533
GGTACGA28200.017.02039318
TGCTCGA33850.017.01524210
CCGACGG41400.016.956747120
TGCGAAT31850.016.95359618
GATGCGA32350.016.6914816
Produced by FastQC (version 0.11.2)