FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20734238
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1067550.5148730327104377No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA450120.21709020606399906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG28200.032.334661
TTGACGG33500.025.06876431
CTACGGT26650.023.40848413
TACGGTG27300.022.8511414
CTAGACG30350.022.139553
GGTACGA29150.020.57912318
GACGGCT40650.020.21667733
TCCGCAA31100.020.05961833
TGACGGC43550.019.97236832
GTACGAG30150.019.89656619
TAGACGC58250.019.6719254
CCTACGG33100.019.39100312
CGGCTAT49050.019.3228435
TAGGACG63700.019.1190364
GTCCGCA35000.018.16718132
CGTCAGA28650.018.007261
TACGAGG33700.017.622820
ACGGCTA49400.017.00015634
GACGCAC38200.016.9611856
ACGGTGA38750.016.71863215
Produced by FastQC (version 0.11.2)