FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20679071
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT474040.22923660352053532Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29050.028.5059831
TCTCGGG51150.023.69578120
CGTATCC136550.023.376808120
TTGACGG35200.022.33113131
TCGCCGG75200.021.942198120
CGGTGGG65900.021.032545120
CCGACGG43700.020.732937120
CGCCGTT26250.020.114935120
GACGGCT42750.017.82582733
CGTAATC33750.017.59933522
CGGCTAT53250.017.23928535
TGACGGC45950.017.2373532
GGTACGA27600.017.17309818
CGTACGT29150.017.08509634
TAGGACG61150.016.9766794
CTACGGT24400.016.96640813
CCGTACG30900.016.50602533
TACGGTG25450.016.50216314
GCGAATT29150.016.26006719
ACGGCTA48900.016.19731334
Produced by FastQC (version 0.11.2)