FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20679071
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1058320.5117831453840456No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA442140.2138103786190395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27050.032.8227041
TTGACGG35850.022.92388531
CTACGGT25900.021.30769313
TACGGTG26900.020.29259114
GGTACGA24000.019.99676318
CCTACGG29300.019.03985812
TAGGACG60650.018.9928554
GACGGCT42450.018.794533
GTACGAG26550.018.75529519
CTAGACG31100.018.7124733
CGAGACC54400.018.526144120
TGACGGC46900.017.65074532
TAGACGC57950.017.6001054
TGCGACC34000.017.46645210
CTGGTCG39400.017.3578059
AACTGCG39800.016.8819757
TCCGCAA32050.016.84500933
GACGCAC34700.016.7700256
CGGCTAT52900.016.55622535
ACGCACC34700.016.2512477
Produced by FastQC (version 0.11.2)