FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20678049
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT469430.2270185161085555Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG53600.024.290827120
CGTCTAG29650.023.2747271
TCGCCGG74450.022.88764120
CGTATCC140550.020.960325120
CGGTGGG63600.020.471516120
CGCCGTT27950.020.39338120
CTACGGT24600.019.75487113
TTGACGG32400.019.26500331
TACGGTG25150.018.84574914
CCGACGG41500.018.795013120
TAGGACG61150.018.0564694
TGCGAAT33400.017.4241518
CTAGACG30700.017.3965473
GTCCTAC121700.016.863471
GCGAATT33350.016.73140319
GGTACGA26750.016.14860718
TCCGCAA29500.016.07196633
GATGCGA34450.016.02222616
ATGCGAA35900.015.87645117
TGACGGC43300.015.662868532
Produced by FastQC (version 0.11.2)