FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20678049
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1053350.5094049250004196No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA441010.2132744728479945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG28250.028.2426831
CTACGGT25300.022.5239413
CTAGACG29500.021.55733
TACGGTG26250.021.48017114
TTGACGG35100.021.36303931
TAGGACG63400.019.68274
TCCGCAA29250.019.07292433
TAGACGC56450.018.7050674
CGAGACC59250.018.628597120
GGGCGTT35750.018.45766633
GACGCAC35800.017.9298086
CGTACGT25250.017.8182634
CCTACGG32400.017.77327512
GTCCGCA31900.017.48849332
CGTCTAA29300.017.4029961
CGTAATC30350.017.19893522
CCGTACG26900.016.72511533
AACTGCG38600.016.6293187
ACGCACC36450.016.6227117
GTATAGG81650.016.457531
Produced by FastQC (version 0.11.2)