FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20656272
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT471250.22813894007592464Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29200.027.5371361
CGCCGTT25700.023.811693120
TCTCGGG51450.023.555332120
TCGCCGG70350.023.452644120
CGTATCC137600.023.065372120
TTGACGG35300.021.08109731
TACGGTG24300.019.75243414
CTACGGT24950.019.47831313
GGTACGA28600.018.46088418
TAGGACG60200.017.8420564
CCGACGG38450.017.63215120
CGGTGGG61350.017.602776120
TCCGCAA28350.017.14660633
AACTGCG36450.017.1200187
GTACGAG30800.016.9486819
GACGGCT44200.016.83626233
CGGCTAT53500.016.59866735
TGACGGC45950.016.3256632
CCTACGG30150.016.31786712
GGTCGGT54250.016.0358711
Produced by FastQC (version 0.11.2)