FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20656272
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1051000.5088042992462531No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA440140.2130781391724509No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27800.031.7225321
CTACGGT23700.025.81733313
TACGGTG25100.024.61685414
TTGACGG33900.022.8257731
TCCGCAA28200.021.0581633
CGAGACC58050.020.253387120
GGTACGA27150.020.1066918
CTAGACG31450.020.0296043
TAGGACG60550.019.419844
GTCCGCA31450.019.26349432
TCGTACG5001.8189894E-1219.19748197
CGTAATC30900.019.02497122
CCTACGG32300.018.94336912
GACGGCT40450.018.53641733
TGACGGC43150.018.4886532
TTGGACG33000.018.3601617
TAGACGC56900.018.2405344
CGTACGT26500.018.10833234
GTACGAG29500.018.09813519
CCGTACG27550.018.0713633
Produced by FastQC (version 0.11.2)