FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20613741
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT471160.22856598421412203Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG51950.024.25205120
CGTCTAG30700.024.240721
CTACGGT24450.022.57528313
TACGGTG25100.021.2743514
CGTATCC144350.021.072008120
CGCCGTT26300.020.75871120
TCGCCGG72700.020.631002120
CTAGACG30800.019.2906423
CGGTGGG66350.019.07903120
TTGACGG34300.018.72230331
CCTACGG29750.018.35179912
CCGACGG42150.018.07677120
TCCGCAA31200.016.92768933
TGCTCGA35650.016.82883810
GCTCGAA33950.016.78802511
TGACGCC61150.016.777004120
GGGCGTT41550.016.75546633
GGTACGA26200.016.71720718
TAGACCG29700.016.1654344
TAGGGCG43400.016.04123731
Produced by FastQC (version 0.11.2)