FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20613741
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1053770.5111978461357403No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA443820.21530298648847873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27900.031.3942851
TTGACGG34050.022.90188631
CGAGACC58650.021.583017120
CTACGGT25800.021.15766713
TACGGTG26100.020.68625314
CTAGACG31450.020.220283
GGTACGA25300.018.73214118
TAGACCG30250.018.2455964
TAGACGC56450.018.0667744
CGGCTAT50150.017.9418635
GACGGCT42800.017.93949333
TAGGACG58950.017.4023514
TGACGGC45400.017.17637432
TCCGCAA30800.017.13862433
CGGTGTA31200.017.112039
GGTCGGT54000.017.10775811
CCTACGG31700.017.03144512
GGGCGTT34650.016.79238933
AACTGCG39350.016.7692667
GTACGAG27650.016.70692419
Produced by FastQC (version 0.11.2)