FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20578533
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT468490.22765957126292727Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27450.027.5495071
TCTCGGG51350.023.249554120
TCGCCGG69000.021.736666120
CCGACGG43100.021.157692120
TTGACGG31850.021.10399431
CGTATCC151400.019.85244120
CGTAATC30550.018.26608722
CGGTGGG65500.018.135347120
CGCCGTT30050.017.968014120
TAGGACG60600.016.539414
TGACGGC42800.016.12538732
AGACCGT37600.016.119666
TACGGTG24600.016.09792714
GACCGTT32150.016.0502097
TTGGACG34400.016.04703117
GACGGCT42350.015.87159933
CGGCTAT48400.015.74714435
CTACGGT24550.015.64030613
GCTCGAA31150.015.60046111
CGTACGT29650.015.5855534
Produced by FastQC (version 0.11.2)