FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20578533
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1047440.5089964381814778No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA441800.21468974489095022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29150.025.518821
CGAGACC59150.022.113008120
TTGACGG35200.019.25626831
CTACGGT21700.019.07412713
CGTACGT25650.018.24078634
CCGTACG26850.018.0957733
ACCGGTA30000.017.59534334
TACGGTG22950.017.51281214
CGTAATC30650.017.026222
GGTACGA28550.016.59805918
TAGGACG60800.016.576054
GGTCGTA27550.016.11153629
TTGGACG32400.016.10692617
ATACGCG40400.016.0368024
GTACGAG30050.015.969151519
TCTGACG23450.015.85995911
TAGACGC57350.015.7949734
TGACGGC44100.015.77813832
GCGAATT30500.015.73354119
TGGTCGG38950.015.70882710
Produced by FastQC (version 0.11.2)