FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20587504
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT465250.2259865984725492Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG50950.024.139305120
CGTCTAG30250.023.8055691
CGCCGTT25500.021.880531120
TCGCCGG72600.021.568453120
CGTATCC153750.021.422607120
TTGACGG33500.020.06163831
CCGACGG43400.019.76794120
GGTACGA26700.019.5508818
CGGTGGG63500.019.273987120
CTACGGT24050.019.20884713
GGTCGGT55700.018.20302211
GTACGAG29650.018.01041419
TAGGACG62150.017.6698424
TACGAGG30100.017.54181520
TACGGTG25800.017.44142714
CTGGTCG44600.017.0836549
TAGACGC58900.016.9127984
GCGAATT32150.016.60991319
CTAGACG32000.016.3151863
ATGCGAA35200.015.85253217
Produced by FastQC (version 0.11.2)