FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20587504
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1047600.5088523601501183No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA438870.2131730004764055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG28000.029.1389871
TTGACGG33150.021.71488631
CGAGACC62100.021.643423120
CTACGGT24950.020.67721213
TACGGTG26150.019.26955214
CTGGTCG42550.018.4680929
TAGGACG64300.018.2859974
CGTAATC31100.017.93825722
TGGTCGG37350.017.827410
CTAGACG31500.017.5206743
AACGGAC27000.017.32922215
TCAACGG28100.017.291913
TCCGCAA29000.017.16879833
CGTACGT27750.017.07748634
GACGGCT38400.017.02766433
TAGACGC58350.016.9635454
TGACGGC43550.016.66698332
ACGGACC28800.016.66323916
AACTGCG39450.016.421447
CAACGGA32000.016.12176114
Produced by FastQC (version 0.11.2)