FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20647046
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT461690.2236106801912487Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT23350.064.9648594
CGACGTA33450.045.34915592
TGTCGGT26200.044.1375493
ACGTATC35900.040.7384594
TCGACGT36900.040.55649691
ACGCCGT31050.038.1195894
TAGATCG28450.036.82332288
CACCGTA41500.036.7164393
GGTCGAC42100.036.0329789
GTCGATC29350.035.9239592
GTGATCG24600.035.39475691
TACGAGT22650.034.53743794
GTCGGTG33300.034.52255694
GTGTCGA30950.034.28697690
TCGGTAG25450.034.21365789
TGATCGC26050.034.20771492
TGGTCGA44600.034.01375288
GTAGTCG25950.033.55313592
CTCGGTA25700.033.35200588
GTGGACG40700.033.09390
Produced by FastQC (version 0.11.2)