FastQCFastQC Report
Mon 11 Dec 2017
B6-M-ATAC-2-4_TCCTGAGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-M-ATAC-2-4_TCCTGAGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20647046
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1027020.4974174029543984No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA432420.2094343181102033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG27900.034.190321
TTGACGG36400.025.37957431
CTACGGT25400.023.38112613
GACGGCT43400.021.14787333
TACGGTG28600.020.76445214
TCCGCAA28200.020.42147633
CGAGACC62650.020.402304120
TGACGGC45350.020.37081532
CTAGACG32200.020.1221283
CCTACGG31200.019.41917412
CGGCTAT54850.018.48333535
TAGACGC59400.017.9782734
GGTCGGT54400.017.75378411
TAGGACG62250.017.637064
GACGCAC35950.017.5207756
GTCCGCA33550.017.34379832
CGTACGT25300.017.30881334
GCGAATT30700.017.00029219
TACGCGT42800.016.9611785
ACGCACC35150.016.8959777
Produced by FastQC (version 0.11.2)