FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23622860
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT280890.11890600884058916Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32600.029.636111
TCTCGGG65750.023.452675120
TTGACGG41750.021.55766531
CGGTGGG93750.021.184183120
CTACGGT26850.020.78102713
CGCCGTT51350.020.331236120
TAGGACG84900.019.578754
TACGGTG28100.019.42957914
CGTATCC191750.018.89977120
CTAGACG32000.017.815033
GGTACGA32400.017.77698918
CGTCTTA30900.017.47480615
GTCCTAC153850.017.1620061
TCGCCGG122200.016.792288120
TACAGTG184050.016.1705135
GGACGTG101650.016.1135396
TCCGCAA36850.015.95715733
CGGCTAT60400.015.59619335
TACGAGG39250.015.59166620
CGTAATC36550.015.43059222
Produced by FastQC (version 0.11.2)