FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23622860
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA266130.11265782381980843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29000.033.9246941
TTGACGG42050.023.25235231
CTACGGT29350.022.27702713
TAGGACG90700.022.1586114
TACGGTG29800.021.7393414
CTAGACG34250.019.968723
GTCCTAC150050.018.6702861
GGACGTG104800.018.3180796
CCTACGG36850.017.90592812
TCTTACG39650.017.7007144
TCCGCAA39000.017.22656633
TAGACGC66800.016.9742414
CGGCTAT58600.016.7879635
CGTAATC36250.016.5484922
TACAGTG188700.016.4052145
GCGTAAA36000.016.3302821
CCGTAAC38000.016.25898429
GCGAATT39150.016.2425219
CGTACGT33250.016.23684534
GACGGCT56050.016.16019433
Produced by FastQC (version 0.11.2)