FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23596156
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT275880.1169173487410407Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG33400.030.3633211
TCTCGGG68600.023.178667120
CGGTGGG88900.021.193104120
TTGACGG43200.021.11275731
CGCCGTT49100.020.652468120
CGTATCC203100.020.414314120
CTACGGT28400.020.06939313
TACGGTG29300.019.0433914
CTAGACG33150.018.6450163
TAGGACG82650.018.2964464
CGTCTTA32350.017.43341615
TCGCCGG123700.017.268198120
GTCCTAC153550.016.726421
TCCGCAA35250.016.68211733
CCGACGG71250.016.25321120
GGTACGA34000.016.234618
TAGACGC68200.016.1018494
CGGCTAT60250.015.83381735
CCTACGG36900.015.77154912
GACGGCT56900.015.607511533
Produced by FastQC (version 0.11.2)