FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23596156
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA261600.11086551555261796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG30700.033.2193871
TAGGACG89750.021.9930634
TTGACGG43550.020.6611631
CTACGGT29100.018.55212813
GTCCTAC150350.018.5138021
GGACGTG106800.018.1436356
TACGGTG30650.017.61393214
GGTACGA32550.017.50902418
TAGACGC66850.017.321294
TCTTACG36200.017.2347144
CTAGACG34150.017.0414033
CGTCTTA39200.016.98622115
CGGCTAT60100.016.96807335
AGACCGT47600.016.5103916
TTAGACG37650.016.094623
GACGGCT55550.015.98193133
GTACGAG37450.015.85972719
TAGACCG35200.015.851294
GTAGGAC160150.015.8091963
TAGGGCG45700.015.35752631
Produced by FastQC (version 0.11.2)