FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23560947
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT277480.11777115750058773Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG30900.026.217441
TCTCGGG65850.023.780827120
TAGGACG88600.020.725394
CGTATCC199550.020.385445120
CGGTGGG88750.019.470016120
TCGCCGG120550.018.216127120
TTGACGG42200.017.49305731
GGACGTG103550.017.267216
CGTCTTA32550.017.14184415
GTCCTAC155100.017.0624661
CGCCGTT44900.016.970692120
GGTACGA35350.015.95386318
CCGACGG69700.015.752899120
TACAGTG182550.015.3499425
TACGGTG27000.014.88800814
CTACGGT26150.014.68364413
AGGACGT148400.014.6368075
TAGATCC234350.014.516444120
CTACAGT152550.014.5154054
GACGGCT53950.014.46151833
Produced by FastQC (version 0.11.2)