FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23560947
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA263870.11199464945105984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29300.033.373151
TTGACGG41600.022.06251731
TAGGACG86950.021.5277294
CTACGGT30100.021.12421813
CTAGACG33300.020.8990693
TACGGTG32300.020.05671314
TAGACGC65600.019.0227224
GTCCTAC150600.017.4472261
GGACGTG104000.017.016156
CCTACGG38100.016.84612712
GTAGGAC160550.016.8157163
TCCGCAA39050.016.12969433
TTAGACG36650.016.042253
CGGCTAT60750.015.8983935
TGACGGC60650.015.627274532
GGTACGA34000.015.350389518
GTCCGCA43400.015.34229132
TGGTCGG56100.015.29018110
CTGGTCG64650.015.1245829
GACGGCT58000.015.10020233
Produced by FastQC (version 0.11.2)