FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23568682
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT276150.11716819803500254Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32950.025.6783081
TCTCGGG69100.024.050611120
CGTATCC196100.021.630484120
TAGGACG91350.020.2314244
CGGTGGG87200.020.159304120
CGCCGTT47550.018.547749120
GTCCTAC152000.018.1995241
TTGACGG42500.017.93336331
TCGCCGG121100.017.785852120
CTACGGT27850.016.80387513
GGACGTG105500.016.5508466
CTACAGT157700.015.5623764
CCGACGG70100.015.491197120
TACAGTG187200.015.0973025
CTAGACG32600.015.0938143
CGTAATC36300.015.04176522
CGGCTAT60250.014.93845335
GTAGGAC160650.014.9037283
CGTACGT34450.014.80660434
CCTACAG170550.014.6719243
Produced by FastQC (version 0.11.2)