FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23568682
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA261170.1108123059235981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29350.033.1129881
TTGACGG41450.020.83864631
TAGGACG91150.020.4037634
CTACGGT29850.020.2969913
TACGGTG30900.019.60782614
CTAGACG34250.018.5673773
TAGACGC68700.018.5133254
GTCCTAC149150.018.0598661
TTAGACG39600.017.5738963
GTAGGAC158100.016.8103663
TAATCGA32250.016.7408521
GGTACGA34350.016.59072318
GGACGTG105900.016.4847036
TCCGCAA37100.016.16801633
TAGACCG33800.016.1521344
GACGGCT52550.015.86619333
CCTACGG38200.015.86041212
TACAGTG184850.015.8067095
ATCGATA35250.015.65645523
GACCGTT38100.015.587717
Produced by FastQC (version 0.11.2)