FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23454387
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT272710.1162724909416733Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG67400.024.834389120
CGTCTAG34500.022.9623761
TAGGACG85450.020.4377774
CGTATCC204400.019.283848120
CTACGGT28850.018.71656813
CGGTGGG87650.018.412365120
TACGGTG29650.018.00994714
TCGCCGG122100.017.73784120
TTGACGG41900.017.47465931
CTAGACG32250.017.3067383
CCGACGG70450.017.287184120
GGACGTG102750.016.5864016
GTCCTAC149550.016.0923671
CGCCGTT46700.016.058403120
TCCGCAA36350.015.35470433
TAGACGC68150.015.14660554
GGTCGGT76300.015.09728411
CGTAATC38800.015.000689522
TAGATCC258150.014.966592120
CGTCTTA33100.014.6826415
Produced by FastQC (version 0.11.2)