FastQCFastQC Report
Mon 11 Dec 2017
B6-F-ATAC-2-3_AGGCAGAA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB6-F-ATAC-2-3_AGGCAGAA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23454387
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA259320.11056353764436477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29800.034.8285261
TTGACGG41750.021.83892331
TAGGACG88800.020.6056614
CTACGGT29500.020.13045513
TACGGTG29450.019.75886714
TAGACGC65450.019.1573894
GGTACGA33900.018.75783318
CGTCTAA37800.018.569461
CTAGACG34300.018.1904933
CGGCTAT60800.017.85774235
TTAGACG36750.017.7940313
GACGGCT54450.017.40613733
GGACGTG105900.017.1650646
GTCCTAC149400.016.785371
TACGAGG38000.016.73492620
GTACGAG38300.016.60384419
CCTACGG37000.016.37524612
CGTAATC34650.016.27453422
GTAGGAC156800.016.2610323
TGACGGC61600.015.87270732
Produced by FastQC (version 0.11.2)